Agile R100 Biosensors

 

Built with breakthrough label-free Field Effect Biosensing (FEB) technology, Agile R100 biosensors help you fully characterize and explore your interactions to make better decisions earlier. The surface of the biosensor is made of graphene, platinum, and glass – biologically inert materials that minimize non-specific binding. Agile R100 biosensors enable accurate kinetic characterization in complex samples such as solvents, detergents, cell lysate, and blood fractions because the electrical sensing mechanism is impartial to optical impediments, providing a novel, orthogonal technique to measure binding.

Agile biosensors are single-sample, regeneration-capable and cost-effective, using just 10 µL of sample.  A variety of biosensors offer convenient surface chemistries for your binding pair and can also be customized for unique experimental conditions and applications.

See the table below to find the biosensor that best suits your needs.

BiosensorChip SurfaceUseConsiderations
NTANTA-functionalized graphene surfaceFor immobilization of his-tagged targets- Enables control of target orientation
- Standard target release protocol for biosensor reuse
NHSAmine-reactive graphene surfaceFor target immobilization via free amine groups- Faster immobilization protocol than COOH
- Covalent linkage of target
SCOOHHigh-density amine-reactive hydrophilic surfaceFor target immobilization via free amine groups- Uses EDC/sNHS
- Covalent linkage of target
- Decreases nonspecific binding from hydrophobic analytes
- 25 times the binding capacity of COOH chips
COOHAmine-reactive graphene surfaceFor target immobilization via free amine groups- Uses EDC/sNHS
- Covalent linkage of target
NH2Carboxyl-reactive graphene surfaceFor target immobilization via free carboxyl groups- Uses EDC/sNHS
- Covalent linkage of target
FLEXUniform hydrophobic graphene surfaceFor customizable immobilization and experiment design- Study membrane fractions and lipid monolayers
- Non-covalent linkage of target

His-tag NTA Agile biosensor chip

Agile R100 His-tag-Immobilization Biosensor Chip – NTA

The Agile R100 His-tag-Immobilization Biosensor Chip – NTA provides for the immobilization of histidine-tagged molecules to the biosensor surface, convenient for a majority of recombinant proteins and allowing you to control the orientation of your immobilized molecule.

NHS Agile biosensor chip

Agile R100 Rapid Amine-immobilization Biosensor Chip – NHS

The Agile R100 Rapid Amine-immobilization Biosensor Chip – NHS has pre-activated amine-linker chemistry that enables you to immobilize your target directly to the biosensor surface. Molecules can be covalently linked to the surface via amine groups, enabling kinetic characterization for antibodies, proteins, peptides, or small molecules.

Amine SCOOH Agile biosensor chip

Agile R100 Super Amine-immobilization Biosensor Chip – SCOOH

The Agile R100 Super Amine-immobilization Biosensor Chip – SCOOH provides a high-density amine-reactive, hydrophilic surface for target immobilization via free amine groups. Specifically designed for high-concentration measurements, SCOOH biosensors decrease background noise caused by hydrophobic analytes.

Amine COOH Agile biosensor chip

Agile R100 Amine-immobilization Biosensor Chip – COOH

The Agile R100 Amine-immobilization Biosensor Chip – COOH is compatible with standard amine-linker chemistry. Molecules can be covalently linked to the biosensor surface via amine groups, enabling kinetic characterization for antibodies, proteins, peptides, or small molecules.

Carboxyl NH2 Agile biosensor chip

Agile R100 Carboxyl-immobilization Biosensor Chip – NH2

The Agile R100 Carboxyl-immobilization Biosensor Chip – NH2 is compatible with standard amine-linker chemistry. Molecules can be covalently linked to the biosensor surface via carboxyl groups, enabling kinetic characterization for antibodies, proteins, peptides, or small molecules.

FLEX Agile biosensor

Agile R100 Hydrophobic-immobilization Biosensor Chip – FLEX

The Agile R100 Hydrophobic-immobilization Biosensor Chip – FLEX provides a hydrophobic surface for customizable surface chemistry, enabling rapid immobilization of most proteins, membrane fractions, and lipid monolayers.