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Q: What reagents are supplied by Nanomedical Diagnostics?
A: The necessary amount of Nanomedical Diagnostics Quench 1 and Quench 2 are included to run measurements on all biosensor chips that are purchased from the company, including the 5 Biosensor Chips included with the AGILE R100 system.
Q: What reagents do I need to supply to run measurements with AGILE R100?
A: Biotechnology grade 2-(N-morpholino)ethanesulfonic acid (MES) buffer pH 6.0, 50 mM.
N-Hydroxysulfosuccinimide (sulfo-NHS; CAS No. 106627‑54‑7).
Ultra High Purity (UHP) grade N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC; CAS No. 25952-53-8).
Culture or molecular grade 1X phosphate buffered saline (PBS) pH 7.4.
Culture or molecular grade deionized water (CAS No. 7732-18-5).
Q: How do I link my capture molecule to the AGILE biosensor chip surface?
A: Current AGILE biosensor chips come with exposed carboxyl groups to covalently link capture molecules directly to the graphene surface. The most common linkage is to use carbodiimide crosslinker chemistry (i.e. EDC/sulfo-NHS). EDC and sNHS activate the carboxyl groups on the surface of the graphene to react with any free amine group in your capture molecule and form a covalent amide bond. Alternatively, His-tag proteins have been immobilized to AGILE biosensor chips, and capture molecule binding with nickel chemistry has been performed on AGILE biosensor chips. Additionally, you can start by using EDC/sulfo-NHS chemistry with a biheterofunctional crosslinker where one functional group is an amine and the other is the functional group favored for your capture molecule of interest (e.g. N‑succinimidyl 3‑(2‑pyridyldithio)propionate (SPDP) for use with thiol‑reactive proteins). This makes AGILE R100 flexible and adaptable to established research and development pipelines. Other binding chemistries are possible, but AGILE biosensor chips have not been thoroughly tested for other chemistries at this time.
Q: How do I learn to use AGILE R100?
A: One day of onsite training with a Nanomedical Diagnostics Field Applications Scientist who can provide training to an unlimited number of attendees on the usage of AGILE R100 is highly recommended. The system also comes with an extensive User Manual for reference.
Q: What kind of experimental design or assay optimization help is offered by Nanomedical Diagnostics?
A: Field Application Scientists are on hand to provide technical support or travel to the site to help with chemistry optimization or other questions related to AGILE R100. Contact technical support to inquire!
Q: How many AGILE R100 Biosensor Chips will I need for my experiment?
A: A single experiment (such as for a dose-response curve of a biological interaction) will require 1 biosensor chip or more, and the number of biosensor chips is dependent on the biological interaction of interest. If the interaction between the capture molecule and target analyte fully dissociates (with or without regeneration techniques), up to 10 interactions can be measured using a single AGILE R100 Biosensor Chip. If the interaction does not fully dissociate, 1 biosensor chip can measure a single interaction, and the number of biosensor chips needed will equal the number of interactions tested.
Q: How many times can I use an AGILE R100 Biosensor Chip?
A: The AGILE biosensor chip can be used for up to 10 measurements, depending on capture molecule stability, analyte stability during the experiment, and success of analyte dissociation from capture molecule.
Q: When I choose transistors in the AGILE software, is the data still being recorded?
A: Yes, when you choose transistors in the AGILE software, the data from every transistor continues to record. Only the data for the selected transistors is displayed, but all data is recorded.
Q: Can I export my data to another software after performing a measurement?
A: The raw data can be exported as a .csv file for further analysis using a preferred scientific graphing and statistics software (such as GraphPad Prism). All images (such as the Experiment Graph, Analysis Data, and Analysis Graphs) can be exported as .jpg files. Additionally, an Experiment Report PDF file can be generated that exports all the data from an experiment file, including the Protocol, Notes, Analysis Settings, Analysis Data, and Analysis Graphs.
Q: What is the difference between direct connect and cable connect?
A: The Direct Connect cartridge connects directly to the AGILE R100 reader for compact use and can be separated from the reader post-experiment for easy decontamination with standard protocols. The Cable Connect cartridge attaches to the reader via a one-meter-long high density cable. This enables the additional use of the cartridge in a wide range of experimental settings (e.g. with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader. The Cable Connect cartridge is also easily decontaminated post-experiment with standard protocols. Both cartridges are compatible with the AGILE reader. While one cartridge is included with the AGILE R100 system purchase, additional cartridges are available as accessories.
Q: Can the Cable Connect cartridge connect directly into the AGILE reader?
A: No, the input connector of the Cable Connect cartridge is slightly different than the Direct Connect cartridge and cannot be plugged directly into the AGILE reader. The Cable Connect cartridge is designed to plug into a high-density cable that is included with the cartridge.
Q: Can the Direct Connect cartridge be connected to the AGILE reader via a high-density cable?
A: No, the input connector of the Direct Connect cartridge is slightly different than the Cable Connect, and cannot be used with a high-density cable. The Direct Connect cartridge is designed to plug directly into the reader for researchers who want the compact convenience of Direct Connect.
Q: What are the dimensions of the system?
A: The AGILE cartridge is approximately (L x W x H) 3.2” x 3” x 1.4”, and the AGILE reader is approximately 5.5” x 3” x 1.4”.
Q: What is the power supply of AGILE R100?
A: AGILE R100 connects to a computer via a USB to micro-USB cable and is powered by the computer. AGILE R100 requires minimal processing power, and a laptop or computer running other applications can power AGILE R100.