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Q: What reagents are supplied by Nanomedical Diagnostics?
A: The necessary amount of Nanomedical Diagnostics Quench 1 and Quench 2 are included to run measurements on all biosensor chips that are purchased from the company, including the 5 Biosensor Chips included with the AGILE R100 system.
Q: What reagents do I need to supply to run measurements with AGILE R100?
A: Biotechnology grade 2-(N-morpholino)ethanesulfonic acid (MES) buffer pH 6.0, 50 mM.
N-Hydroxysulfosuccinimide (sulfo-NHS; CAS No. 106627‑54‑7).
Ultra High Purity (UHP) grade N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC; CAS No. 25952-53-8).
Culture or molecular grade 1X phosphate buffered saline (PBS) pH 7.4.
Culture or molecular grade deionized water (CAS No. 7732-18-5).
Q: How do I immobilize my target to the AGILE biosensor chip surface?
A: Current AGILE biosensor chips come with exposed carboxyl groups to covalently link target directly to the graphene surface. The most common linkage is to use carbodiimide crosslinker chemistry (i.e. EDC/sulfo-NHS). EDC and sNHS activate the carboxyl groups on the surface of the graphene to react with any free amine group in your target and form a covalent amide bond. Alternatively, His-tag proteins have been immobilized to AGILE biosensor chips, and immobilizing the target using nickel chemistry has been performed on AGILE biosensor chips. Additionally, you can start by using EDC/sulfo-NHS chemistry with a biheterofunctional crosslinker where one functional group is an amine and the other is the functional group favored for your target of interest (e.g. N‑succinimidyl 3‑(2‑pyridyldithio)propionate (SPDP) for use with thiol‑reactive proteins). This makes AGILE R100 flexible and adaptable to established research and development pipelines. Other binding chemistries are possible, but AGILE biosensor chips have not been thoroughly tested for other chemistries at this time.
Q: How do I learn to use AGILE R100?
A: One day of onsite training with a Nanomedical Diagnostics Field Applications Scientist who can provide training to an unlimited number of attendees on the usage of AGILE R100 is highly recommended. The system also comes with an extensive User Manual for reference.
Q: What kind of experimental design or assay optimization help is offered by Nanomedical Diagnostics?
A: Field Application Scientists are on-hand to provide technical support or travel to the site to help with chemistry optimization or other questions related to AGILE R100. Contact technical support to inquire!
Q: How many AGILE R100 Biosensor Chips will I need for my experiment?
A: A single experiment (such as for a dose response curve of a biological interaction) will require 1 biosensor chip or more, and the number of biosensor chips is dependent on the biological interaction of interest. If the interaction between the immobilized target and analyte in solution fully dissociates (with or without regeneration techniques), up to 10 interactions can be measured using a single AGILE R100 Biosensor Chip. If the interaction does not fully dissociate, 1 biosensor chip can measure a single interaction, and the number of biosensor chips needed will equal the number of interactions tested.
Q: How many times can I use an AGILE R100 Biosensor Chip?
A: The AGILE biosensor chip can be used for up to 10 measurements, depending on the stability of the immobilized target, stability of the analyte during the experiment, and success of analyte dissociation from the immobilized target.
Q: When I choose transistors in the AGILE software, is the data still being recorded?
A: Yes, when you choose transistors in the AGILE software, the data from every transistor continues to record. Only the data for the selected transistors is displayed, but all data is recorded.
Q: Can I export my data to another software after performing a measurement?
A: The experimental data can be exported as a .csv file for further analysis using a preferred scientific graphing and statistics software (such as GraphPad Prism). All images (such as the Experiment Graph, Analysis Data, and Analysis Graphs) can be exported as .jpg files. Additionally, an Experiment Report PDF file can be generated that exports all the data from an experiment file, including the Protocol, Notes, Analysis Settings, Analysis Data, and Analysis Graphs.
Q: What is the VHD Cable and how do I use it?
A: The AGILE R100 cartridge can connect directly to the reader, and the very high density (VHD) cable accessory provides the option of connecting the cartridge either directly to the reader or connecting using the VHD cable. If you have the VHD cable accessory, you have the additional option of using the cartridge in a wide range of experimental settings (e.g. with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader. The cartridge and VHD cable can be separated from the reader post-experiment for easy decontamination with standard protocols.
Q: What are the dimensions of the system?
A: The AGILE cartridge is approximately (L x W x H) 3.2” x 3” x 1.4”, and the AGILE reader is approximately 5.5” x 3” x 1.4”.
Q: What is the power supply of AGILE R100?
A: AGILE R100 connects to a computer via a USB to micro-USB cable and is powered by the computer. AGILE R100 requires minimal processing power, and a laptop or computer running other applications can power AGILE R100.