Label-free Agile R100 kinetic binding data assay


Information-rich kinetic binding data lets you understand measures critical to determining clinical efficacy. Agile R100 is a novel label-free orthogonal assay for small molecule and protein hit validation that can detect interactions in complex solutions with just a 10 µL drop of sample. Characterize your candidates earlier in the process – and reduce costly mistakes further down the line.

Easily gain sensitive label-free data including:

  • Kinetic binding data and analysis
  • Affinity ranking
  • Dissociation rates
  • Concentration measurements


  • Complex sample use enables detection in up to 10% DMSO, cell or tissue lysate, and cell growth supernatant.
  • Microfluidics-free design means no clogging or maintenance that requires annoying service contracts.
  • Single-sample format lets you modify your assay mid-experiment.
  • Reusable biosensor chip provides up to 10 measurements.
  • Easy-to-learn instrument requires minimal training.
  • Agile Plus software makes running assays and data analysis easy.
AGILE R100 label-free biophysical assay
AGILE R100 applications


Kinetic binding data at the early stages of drug discovery can provide advance insight into the biological activity profile of your compounds. Easily run assays at your bench in a free afternoon to characterize lead compounds and make confident decisions regarding your candidates.

Choose one of the below applications to learn more:


AGILE R100 for small molecule detectionSmall Molecule

Accurately detect molecules > 10 Da.

AGILE R100 has a small footprintSmall Footprint

System fits in your backpack.

AGILE R100 sensorgram High Sensitivity

11 logs of dynamic range starting at 100 fM.

drobletSmall Volumes

Just 10 µL of sample.
0.5 ng of immobilized target.

AGILE R100 for fast measurementsFast

Get results in an afternoon
on your own schedule.

Measure in DMSOOptically Imperfect Samples

Detect in DMSO, cell or tissue lysate,
or cell growth supernatant.


Graphene AGILE R100 biosensor chip

Biosensor Chips

Built with breakthrough Field Effect Biosensing (FEB) technology, Agile R100 biosensor chips help you fully characterize and explore your interactions to make better decisions earlier. The surface of the biosensor chip is made of graphene, platinum, and glass – biologically inert materials that do not react with your experiment and cause unnecessary nonspecific binding.

Each biosensor chip is built with graphene transistors which provide a minimum of 3 replicate data points per measurement for strong internal reproducibility and technical control. Depending on the stability of the immobilized molecule, each chip can provide 10 measurements.

Label-free Agile R100 cartridge


The Agile R100 reader (not shown) houses the majority of the system’s electronic components. The Agile R100 cartridge holds the biosensor chip and biological materials. It is easy to clean, and an included clear cover reduces liquid evaporation.

The cartridge can connect directly to the reader for compact use and can be separated from the reader post-experiment for easy decontamination with standard protocols.

AGILE R100 cartridge with an optional cable accessory

The cartridge can also attach to the reader via a one-meter-long high density cable, which is an optional accessory. This enables additional use of the cartridge in a wide range of experimental settings (ex: with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader.

Label-free kinetic binding assay Agile R100 reader


The Agile R100 reader houses the majority of the platform’s electronic components and powers the system via a USB connection to a computer or laptop. The reader can connect directly to the cartridge for compact use or be separated via a one-meter-long high density cable, which is an optional accessory. This enables a wide range of experimental settings (ex: with a temperature controlled incubator or in a biosafety cabinet) by providing the ability to use the cartridge in conditions that may otherwise damage the reader.

AGILE R100 label-free kinetic data training


We are committed to helping you capture the best data possible with your Agile R100 platform. Highly-trained and qualified Field Applications Scientists partner with you at your lab or facility to train any size party on how to use Agile R100 to generate high-quality kinetic binding and affinity data at your bench.

The agenda is customizable to your needs, and topics can include the basics of Field Effect Biosensing (FEB) technology, assay development with Agile R100, data acquisition and analysis software use, analysis interpretation, maintenance, cleaning, and troubleshooting.


See more about How It Works.
Agile R100 label-free kinetic binding assay association and dissociation
what does an AGILE R100 FEB sensorgram look like
Dynamic Range of Detection100 fM - 10 mM
Sample Volume10-50 uL
Functionalization Amount0.5-500 ng of material
Temperature Range-20C to 100 C
Lower Sense of Time2 minutes
Upper Sense of Time8+ hours
Size of Immobilized Target1 Da-200 kDa
Size of Analyte in Solution1 Da-200 kDa
Sample TypeSmall molecules in ≤ 10% DMSO, cell & tissue lysate, blood fractions
Target ChemistryEDC/sNHS or His-tag linkage
Speed of Data CollectionUp to 420 points/min
Shelf Stable3-6 months, stored with desiccant
Number of MeasurementsUp to 10 per Agile biosensor chip
Computer SpecificationsPC with USB port, Windows 7 or later
Weight2 pounds
Product Dimensions9 in. x 3 in.


Comparison Studies

Agile R100 versus MicroCal iTC200 Kinetic Binding Data


Agile R100 is a graphene biosensor with unprecedented functionality, providing label-free, real-time kinetic binding data. As a novel technology, Agile R100 is compared to MicroCal iTC200 to demonstrate its benefits compared to a standard kinetic binding analysis tool, isothermal titration calorimetry (ITC). In this study, kinetic binding data between the two instruments is compared by measuring Rho guanosine triphosphate hydrolase enzyme (GTPase) interacting with GTPase activating protein (GAP). The association and dissociation binding rate (ka and kd, respectively) and dissociation constants (KD) are reported. Agile R100 has a precise binding affinity measurement (KD = 1.35 ± 0.06 µM), comparable to MicroCal iTC200 (KD = 2.7 ± 0.3 µM), while also obtaining ka and kd values. Additionally, Agile R100 uses up to 30 million times less sample material and up to 30 times less sample volume and can perform 2.5 times more measurements in a given workday compared to MicroCal iTC200.

Application Notes

Detecting Interactions of Small Molecules in DMSO Using Agile R100


Small molecules are beneficial as drug therapeutics but often have low solubility in aqueous solutions. Solvents such as dimethyl sulfoxide (DMSO) are necessary additives for small molecule solubility, but these solvents can interfere with optical biosensing platforms. In this technical note, Agile R100 successfully detects the interaction between the cytokine tumor necrosis factor alpha (TNFα) and the small molecule inhibitor SPD304 in 0%, 1%, 3%, and 10% DMSO. Because Agile R100 uses an electrical technique rather than an optical one, DMSO in buffer is compatible with the instrument and does not require solvent correction. Additionally, high concentrations of SPD304 (100 μM) were detected on Agile R100. Importantly, Agile R100 detects the association binding rate (ka) and the dissociation binding rate (kd) and can calculate the dissociation constant (KD) of the TNFα and SPD304 interaction in buffer with DMSO. Agile R100’s KD result (14.5 ± 1.8 μM in phosphate buffer saline [PBS] with 10% DMSO) is similar to a previously reported KD value (7.3 ± 0.5 μM in citrate phosphate buffer with 10% DMSO).

Affinity Ranking of SPD304, Evans Blue, and Trypan Blue to TNFα Using Agile R100


Affinity ranking is critical to optimizing the relationship between a target protein and a potential drug compound during the hit-to-lead optimization phase of drug discovery. In this application note, Agile R100 characterizes the activity of the target protein TNFα interacting with the small molecule drug compounds, SPD304, Evans Blue, and Trypan Blue in order to rank order by affinity.