AGILE R100 biosensor applications

AGILE R100

Make more informed decisions earlier in the drug discovery process with sensitive label-free data at your fingertips including:

  • Kinetic binding data and analysis
  • Affinity ranking
  • Dissociation rates
  • Concentration measurements

EASY LABEL-FREE BIOPHYSICAL DATA

The power of real-time kinetic binding data is back in your hands with Agile R100, a novel orthogonal assay for small molecule and biotherapeutic drug discovery. Based on breakthrough Field Effect Biosensing (FEB) technology, Agile R100 detects interactions in optically complex samples using small volumes, giving you control over the complete characterization of your biomolecules earlier in the process – when you need it most!

Agile R100 features:

  • Complex sample use that enables detection in up to 10% DMSO.
  • Microfluidic-free design that means no clogging or dead volume.
  • Simple open-pipetting platform that enables on-the-fly assay modification.
  • Reusable biosensor chips which can be regenerated up to 10 times a chip.
  • Easy-to-learn instrument that requires minimal training
  • Automated real-time analysis software that enables instantaneous readouts of binding data.
AGILE R100 label-free biophysical assay

APPLICATIONS

Agile R100 provides high-quality, reliable data across a range of applications.

  • Small molecule or fragment hit validation and lead optimization
  • Biotherapeutic drug discovery
  • Antibody characterization and development
  • Biomolecular analysis of aptamers, peptides, proteins
AGILE R100 applications

GENERATE KINETIC BINDING DATA AT YOUR BENCH

AGILE R100 hit-to-lead and lead optimization for drug discovery

BENEFITS

AGILE R100 for small molecule detectionSmall Molecule

No lower size limit

AGILE R100 has a small footprintSmall Footprint

Benchtop system weighs only 2 lbs.

AGILE R100 sensorgram High Sensitivity

11 logs of dynamic range starting at 100 fM

drobletSmall Volumes

Just 10 µL of sample
0.5 ng of immobilized target

AGILE R100 for fast measurementsFast

Get results in less than a day
on your own schedule

Measure in DMSOOptically Imperfect Samples

Detect in cell or tissue lysate,
blood fractions, or DMSO

WORKFLOW AND SENSORGRAM

See more about How It Works.
How AGILE R100 works association and dissociation
what does an AGILE R100 FEB sensorgram look like

COMPONENTS

Graphene biosensor AGILE R100 chip

Biosensor Chips

Built with breakthrough Field Effect Biosensing (FEB) technology, Agile R100 biosensor chips help you fully characterize and explore your interactions to make better decisions earlier. The surface of the biosensor chip is made of graphene, platinum, and glass – biologically inert materials that do not react with your experiment and cause unnecessary nonspecific binding.

Each biosensor chip is built with graphene transistors which provide a minimum of 3 replicate data points per measurement for strong internal reproducibility and technical control. Depending on the stability of the immobilized molecule, each chip can be regenerated up to 10 times, providing 10 measurements a chip.

Cartridge

The Agile R100 reader (not shown) houses the majority of the system’s electronic components. The Agile R100 cartridge holds the biosensor chip and biological materials. It is easy to clean, and an included clear cover reduces liquid evaporation.

The cartridge can connect directly to the reader for compact use and can be separated from the reader post-experiment for easy decontamination with standard protocols.

The cartridge can also attach to the reader via a one-meter-long high density cable, which is an optional accessory. This enables additional use of the cartridge in a wide range of experimental settings (ex: with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader.

Reader

The Agile R100 reader houses the majority of the platform’s electronic components and powers the system via a USB connection to a computer or laptop. The reader can connect directly to the cartridge for compact use or be separated via a one-meter-long high density cable, which is an optional accessory. This enables additional use of the cartridge in a wide range of experimental settings (ex: with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader.

AGILE R100 training

Training

We are committed to helping you capture the best data possible with your Agile R100 platform. Highly-trained and qualified Field Applications Scientists partner with you at your lab or facility to train any size party on how to use Agile R100 to generate high-quality kinetic binding and affinity data at your bench.

The agenda is customizable to your needs, and topics can include the basics of Field Effect Biosensing (FEB) technology, assay development with Agile R100, data acquisition and analysis software use, analysis interpretation, maintenance, cleaning, and troubleshooting.

SPECIFICATIONS
Dynamic Range of Detection100 fM - 10 mM
Sample Volume10-50 uL
Functionalization Amount0.5-500 ng of material
Temperature Range-20C to 100 C
Lower Sense of Time2 minutes
Upper Sense of Time8+ hours
Size of Capture Molecules1 Da-200 kDa
Size of Target Analyte1 Da-200 kDa
Sample TypeSmall molecules in ≤ 10% DMSO, cell & tissue lysate, blood fractions
Target ChemistryEDC/sNHS or His-tag linkage
Speed of Data CollectionUp to 420 points/min
Shelf Stable3-6 months, stored with desiccant
Number of MeasurementsUp to 10 per Agile biosensor chip
Computer SpecificationsPC with USB port, Windows 7 or later
Weight2 pounds
Product Dimensions9 in. x 3 in.