EASY LABEL-FREE BIOPHYSICAL DATA

The power of real-time kinetic binding data is back in your hands with AGILE R100, a novel orthogonal assay for small molecule and fragment-based drug discovery. Based on breakthrough Field Effect Biosensing (FEB) technology, AGILE R100 detects small molecules and fragments with no lower size limit, ≤10% DMSO using as little as 10 µL, giving you control over the complete characterization of your biomolecules.

Accelerate your research with a novel approach for hit validation and lead optimization!

AGILE R100 label-free biophysical assay
AGILE R100 biosensor applications

AGILE R100

Make more informed decisions earlier in the drug discovery process with sensitive biophysical data at your fingertips including:

  • Kinetic binding data and analysis
  • Dose-response curves in hours
  • Affinity and concentration measurements
  • Dissociation rates

APPLICATIONS

AGILE R100 provides high-quality, reliable data across a range of applications.

  • Small molecule or fragment hit validation and lead optimization
  • Antibody characterization and development
  • Biomolecular analysis of aptamers, peptides, proteins
AGILE R100 applications

GENERATE KINETIC BINDING DATA AT YOUR BENCH

AGILE R100 hit-to-lead and lead optimization for drug discovery

BENEFITS

AGILE R100 for small molecule detectionSmall Molecule

No lower size limit

AGILE R100 has a small footprintSmall Footprint

Benchtop system weighs only 2 lbs.

AGILE R100 sensorgram High Sensitivity

11 logs of dynamic range starting at 100 fM

drobletSmall Volumes

Just 10 µL of sample
0.5 ng of capture molecule

AGILE R100 for fast measurementsFast

Get results in less than a day
on your own schedule

Measure in DMSOOptically Imperfect Samples

Detect in cell or tissue lysate,
blood fractions, or DMSO

WORKFLOW AND SENSORGRAM

See more about How It Works.

How AGILE R100 works association and dissociation
what does an AGILE R100 FEB sensorgram look like

COMPONENTS

Graphene biosensor AGILE R100 chip

Biosensor Chips

 

With breakthrough all-electronic graphene-based technology, AGILE R100 Biosensor Chips help you fully characterize and explore your biomolecules to make better decisions earlier. The surface of the biosensor chip is made of graphene, platinum, and glass – biologically inert materials that will not react with your experiment.

Each biosensor chip is built with graphene transistors which provide a minimum of 10 replicate data points per measurement for strong internal reproducibility and technical control. Depending on the stability of the immobilized molecule, each chip can be regenerated up to 10 times, providing 10 measurements a chip.

Cartridges

 

The AGILE R100 reader (not shown) houses the majority of the system’s electronic components. The cartridge holds the biosensor chip and biological materials. It is easy to clean, and an included clear cover reduces liquid evaporation.

The Direct Connect cartridge connects directly to the AGILE R100 reader for compact use and can be separated from the reader post-experiment for easy decontamination with standard protocols.

The Cable Connect cartridge attaches to the reader via a one-meter-long high density cable. This enables additional use of the cartridge in a wide range of experimental settings (ex: with a temperature controlled incubator or in a biosafety cabinet) that may otherwise damage the reader. The Cable Connect cartridge is also easily decontaminated post-experiment with standard protocols.

Both cartridges are compatible with the AGILE reader. While one is included with the AGILE R100 system purchase, additional cartridges are available as accessories.

AGILE R100 training

Training

 

We are committed to helping you capture the best data possible with your AGILE R100 platform. Highly-trained and qualified Field Applications Scientists partner with you at your lab or facility to train any size party on how to use AGILE R100 to generate high-quality kinetic binding and affinity data at your bench.

The agenda is customizable to your needs, and topics can include the basics of AGILE technology, assay development with AGILE R100, data acquisition and analysis software use, analysis interpretation, maintenance, cleaning, and troubleshooting.

SPECIFICATIONS
Dynamic Range of Detection100 fM - 10 mM
Sample Volume10-50 uL
Functionalization Amount0.5-500 ng of material
Temperature Range-20C to 100 C
Lower Sense of Time2 minutes
Upper Sense of Time8+ hours
Size of Capture Molecules1 Da-200 kDa
Size of Target Analyte1 Da-200 kDa
Sample TypeSmall molecules in ≤ 10% DMSO, cell & tissue lysate, blood fractions
Target ChemistryEDC/sNHS or His-tag linkage
Speed of Data CollectionUp to 420 points/min
Shelf Stable3-6 months, stored with desiccant
Number of MeasurementsUp to 10 per AGILE biosensor chip
Computer SpecificationsPC with USB port, Windows 7 or later
Weight2 pounds
Product Dimensions9 in. x 3 in.