Small Molecule Detection
Based on breakthrough FEB Technology, AGILE R100 monitors small molecules with no lower size limits.
Data from AGILE R100 showing standard curve of 0.1 – 1000 µM of small molecule PIC in 1X PBS buffer with 1% DMSO binding to immobilized SYK.
Data from AGILE R100 showing standard curve of 0.5 – 100 µM of small molecule SPD304 in 1X PBS buffer with 10% DMSO binding to immobilized TNFα.
Standard curve of small molecule naltrexone binding to immobilized GPCR. Data taken at University of Pennsylvania with a LOD of 10 pg/mL and was validated by ELISA with a LOD of 1 ng/mL. Lerner et al., ACS NanoLetters 2014.
Measurements in DMSO and in High Concentration
Non-optical technology enables AGILE R100 to detect in DMSO without need for solvent correction, reducing time to data.
High Sensitivity Sensing in the Femtomolar Range
AGILE biosensor chips are made with graphene, a single atomic layer of carbon. Every atom of graphene is exposed to the environment, making it highly sensitive to the change in conductance caused by interaction or binding that occurs on the sensor surface. This enables detection even with small amounts of starting material, small volumes, low concentrations, and weak biomolecular interactions.
Data taken with AGILE platform using 500 pg/mL bacterial antigen binding to immobilized monoclonal antibody.
AGILE R100 is designed to use small volumes. The microfluidic-free liquid handling design requires only 10 µL to measure. The optimized binding chemistry means that there is no need to coat the sensor surface with excess protein; only a few thousand proteins are needed for functionalization, enabling nM concentrations and low volumes of material.
Small Material Amount: 10.2 nM
Small Sample Volume Amount: 4 nL
Fast Time to Results
Simple-step, well-based design enables kinetic binding data within minutes of sample prep, ideal for measuring unstable compounds.
Data from AGILE R100 showing recombinant kinase in PBS buffer binding to an immobilized peptide therapeutic.