Field Effect Biosensing (FEB) is a breakthrough label-free technology for measuring biomolecular interactions

FEB Technology

At the heart of Agile R100 is a graphene biosensor chip built with proprietary label-free Field Effect Biosensing (FEB) technology. FEB is an electrical technique that measures the current across a field effect graphene biosensor functionalized with immobilized biomolecular targets (Figure 1). Any interaction or binding that occurs on the surface causes a change in conductance that is monitored in real-time (Figure 2), enabling accurate kinetic, affinity, and concentration measurements. FEB is a unique orthogonal technology that lets you have direct visibility into the innate electrical signaling that occurs in biological systems without translations from optical data.

Field Effect Biosensing (FEB) technology image

Only molecules binding to or dissociating from the biosensor surface cause a change in conductance on the Agile R100 platform. Unbound molecules or crude media that might interfere with optics do not affect the conductance reading. This robustness enables detection in complex samples from solvents and detergents to cell fractions and tissue lysate, making FEB a powerful technology for small molecule or protein drug discovery research.

Small Molecule Detection with an FEB system

FEB is fundamentally different from optical techniques such as SPR or BLI. Optical systems measure shifts in light caused by changes in mass, which is effective for large molecules. SPR and BLI struggle to measure interactions <1000 Da without solvent correction and additional calculations.

In contrast, FEB is an electrical technique, not a mass-based method. The size of the molecule does not impact the change in conductance experienced when an interaction occurs, enabling sensitive detection of biomolecules >10 Da. This makes FEB an excellent orthogonal technique for small molecule characterization and hit validation.

ComparisonFEBBLISPR
No microfluidics - no clogging or set association phasecheckcheckno
Single step reading - fast response, less timecheckcheckno
No impacts due to change in refractive index of surrounding mediumcheckcheckno
No need for solvent correction – reduce number of measurementschecknono
Ability to detect >10 Dachecknono
Small, personal assay systemchecknono