Protein-Protein Interactions

The understanding of protein-protein interactions is vital to the understanding of disease mechanisms and potential therapeutics. Field Effect Biosensing (FEB) is a label-free technique that enables protein characterization without the use of labels that can produce unwanted interactions that can lead to false conclusions. FEB biosensors, such as Agile R100, eliminate the need for tags, dyes, or other specialized materials, reducing resources needed for assay development and simplifying assay design. Easily characterize the interactions you need to translate in vitro biomolecular activity to in vivo drug efficacy.

Agile R100 features:

  • High-quality quantitative kinetic analysis including, kon, koff, and KD
  • Detection in cell or tissue lysate, serum, or DMSO
  • High sensitivity down to 100 fM, enabling detection of tight-binding compounds
  • Fast measurements that enable quick kinetic characterization of unstable proteins
  • Just a 10 µL drop of sample enables more data with less material
  • Regenerate each biosensor chip up to 10 times
label-free Agile R100 dose-response curve
Label-free Agile R100 protein-protein characterization

The figures above show binding activity and selectivity of peptides in vitro, with data taken by Agile R100. The first figure shows the selectivity of dPKC and ePKC binding to yMARCKS-Cargo. The second figure shows a dose-response curve of dPKC binding to yMARCKS-Cargo. Additional info can be found in the Qvit publication linked below.

Additional Literature: