TIGHT BINDERS
Frustrated because the limit of detection (LOD) of your interaction is lower than your instrument can read? Agile R100 has a dynamic range starting at 100 fM so you never have to be hampered by lack of sensitivity again.
Agile R100 Features:
- LLOD in fM range to capture tight binding interactions.
- Trouble-shoot antibody-based assays.
- 8+ hour run-time allows for long dissociation measurements for accurate off-rates.
- Orthogonal electrical sensing mechanism to cross-check interactions.
- Transfer ELISA protocol directly to Agile R100 with little to no assay adjustment.
Immobilized Antibody and IL-6
Data from Agile R100 showing 0.2 – 10,000 pg/mL IL-6 in 1X PBS buffer binding to an immobilized antibody compared to an ELISA. No assay adjustment was made in the transition from ELISA to Agile R100.
Learn More
- Brite Paper: Publication Highlight: 10x More Sensitive than BLI or ELISA
- Technical Note: Calculating Dissociation Rates of Tight Binders Using kobs
- Application Note: Reproducible Kinetic Characterization of Small Molecule Compounds in Serum with Agile R100
- Primer: Intro to Kinetic Binding Data Guide
LEARN MORE:
- Brite Paper: Publication Highlight: 10x More Sensitive than BLI or ELISA
- Technical Note: Calculating Dissociation Rates of Tight Binders Using kobs
- Application Note: Reproducible Kinetic Characterization of Small Molecule Compounds in Serum with Agile R100
- Primer: Intro to Kinetic Binding Data Guide